Library screening resources

Resources for pooled CRISPR library screens:

  1. Cas13 guide RNA design (web app)
    • Tutorial for custom guide design to target and score SARS-CoV-2 (COVID-19) RNA genome (git readme)
  2. GUIDES tool for custom Cas9 library design (web app)
  3. GeCKOv2 libraries (human and mouse) details and availability (Addgene human and mouse)
    • See the Addgene pages for a list of guide sequences in each library and FASTQ sequence data for plasmid pools
  4. Human and mouse non-targeting guides from GeCKOv2 library (xls)
  5. GeCKO (v1 and v2) readout primers (July 2014) (xls)
  6. At-the-bench short protocols:
    • Cloning individual lentiviral CRISPR constructs (pdf)
    • GeCKOv2 library amplification (pdf)
  7. CRISPR Forum — lots of great screening-related FAQs (Google Group)