Library screening resources

Resources for pooled CRISPR library screens:

  1. GUIDES tool for custom library design (web app)
  2. GeCKOv2 libraries (human and mouse) details and availability (Addgene human and mouse)
    • See the Addgene pages for a list of guide sequences in each library and FASTQ sequence data for plasmid pools
  3. Human and mouse non-targeting guides from GeCKOv2 library (xls)
  4. GeCKO (v1 and v2) readout primers (July 2014) (xls)
  5. At-the-bench short protocols:
    • Cloning individual lentiviral CRISPR constructs (pdf)
    • GeCKOv2 library amplification (pdf)
  6. CRISPR Forum — lots of great screening-related FAQs (Google Group)